Journal
/
Neuroscience
/
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

English

Automatically Generated

10,032 Views

•

09:05 min

•

February 09, 2020

DOI:

10.3791/60912-v

DOI:
10.3791/60912-v

•

09:05 min
February 09, 2020

•

3 Views
, , , , ,
1Sorbonne Université, Inserm, CNRS, UMR7225,Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris,GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

Summary

Automatically generated

Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

Explore More Videos

OligodendrocytesOligodendrocyte-conditioned MediumCo-cultureMultiple SclerosisMyelinationCell CultureCerebral CorticesEnzymatic DigestionCell Filtration

Read Article

Cite this Article

Mazuir, E., Dubessy, A., Wallon, L., Aigrot, M., Lubetzki, C., Sol-Foulon, N. Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments. J. Vis. Exp. (156), e60912, doi:10.3791/60912 (2020).

Download .ris file

Copy

Share Video

.

Copy