Journal
/
Genetics
/
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

English

Automatically Generated

5,833 Views

•

10:49 min

•

March 06, 2020

DOI:

10.3791/60654-v

DOI:
10.3791/60654-v

•

10:49 min
March 06, 2020

•

1 Views
, , , ,
1Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Institut de Recherches Cliniques de Montréal (IRCM), 3Département de médecine, Faculté de médecine,Université de Montréal

Summary

Automatically generated

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

Explore More Videos

Transcriptional ControlPlasmid Expression SystemMRNA StabilityPrimary Alveolar Epithelial CellsTransient TransfectionResponse PlasmidGene Of InterestV5 Epitope3′ UTRRestriction AnalysisSequencingPrimary Rat Lung Type II Alveolar Epithelial CellsCell CultureTransfection

Read Article

Cite this Article

Migneault, F., Gagnon, F., Brochiero, E., Berthiaume, Y., Dagenais, A. Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells. J. Vis. Exp. (157), e60654, doi:10.3791/60654 (2020).

Download .ris file

Copy

Share Video

.

Copy