Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault; Fr&#233;d&#233;ric Gagnon; Emmanuelle Brochiero; Yves Berthiaume; Andr&#233; Dagenais

doi:10.3791/60654

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Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

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JoVE Journal 遺伝学

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

5,832 Views

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10:49 min

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March 06, 2020

DOI:

10.3791/60654-v

DOI:

10.3791/60654-v

•

10:49 min

March 06, 2020

•

1 Views

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine,Université de Montréal

概要

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

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Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

概要

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