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Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
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JoVE 杂志 遗传学
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

5,833 Views

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10:49 min

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March 06, 2020

DOI:

10.3791/60654-v

DOI:
10.3791/60654-v

•

10:49 min
March 06, 2020

•

1 Views
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1Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Institut de Recherches Cliniques de Montréal (IRCM), 3Département de médecine, Faculté de médecine,Université de Montréal

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Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

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Migneault, F., Gagnon, F., Brochiero, E., Berthiaume, Y., Dagenais, A. Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells. J. Vis. Exp. (157), e60654, doi:10.3791/60654 (2020).

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