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Genetics

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JoVE Journal Genetics
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
 

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Article DOI: 10.3791/60654-v • 10:49 min • March 6th, 2020
March 6th, 2020 •
1,2,3, 2,3, 1,3, 2,3, 2,3
1Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), 2Institut de Recherches Cliniques de Montréal (IRCM), 3Département de médecine, Faculté de médecine, Université de Montréal

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Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

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Transcriptionally Controlled Plasmid Expression System Investigation Stability Of MRNA Transcripts Primary Alveolar Epithelial Cells Post-transcriptional Modulation Transient Transfection Cell Physiology Metabolism Transcription Inhibitors Response Plasmid Gene Of Interest Multiple Cloning Sites Recognition Sequences High Fidelity Taq Polymerase Overlap PCR Techniques Designer Primers V5 Epitope Mutants Three Prime Untranslated Regions Polyadenylation Site
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