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Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

5,749 Views

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08:15 min

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November 15, 2019

DOI:

10.3791/60470-v

DOI:

10.3791/60470-v

•

08:15 min

November 15, 2019

•

2 Views

Tânia Martins Garcia¹, Marit Navis¹, Manon E. Wildenberg¹, Ruurd M. van Elburg², Vanesa Muncan¹

¹Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M,University of Amsterdam, ²Department of Pediatrics, Amsterdam UMC,University of Amsterdam

Summary

This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.

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