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Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
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発生生物学
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JoVE Journal 発生生物学
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

DOI:
10.3791/60470-v

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08:15 min

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November 15, 2019

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, , , ,
1Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M,University of Amsterdam, 2Department of Pediatrics, Amsterdam UMC,University of Amsterdam

章

  • 00:04標題
  • 00:52Isolation of Fetal Small Intestinal Organoids
  • 03:21Culturing of Fetal Organoids and Maturation Analysis at RNA and Protein Level
  • 04:42Enzyme Activity Analysis
  • 05:52Results: Fetal Organoid Culture
  • 07:22Conclusion

概要

自動翻訳

自動翻訳

This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.

タグ

Fetal Intestinal OrganoidsSuckling-to-weaning TransitionGut MaturationIntestinal CryptsExtracellular Matrix GelENR MediumIn Vitro Gut Model

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