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Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
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Biologie du développement
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Journal JoVE Biologie du développement
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

5,749 Views

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08:15 min

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November 15, 2019

DOI:

10.3791/60470-v

DOI:
10.3791/60470-v

•

08:15 min
November 15, 2019

•

2 Views
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1Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M,University of Amsterdam, 2Department of Pediatrics, Amsterdam UMC,University of Amsterdam

Summary

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This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.

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Cite this Article

Garcia, T. M., Navis, M., Wildenberg, M. E., van Elburg, R. M., Muncan, V. Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids. J. Vis. Exp. (153), e60470, doi:10.3791/60470 (2019).

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