Journal
/
Genetics
/
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

English

Automatically Generated

7,820 Views

•

09:10 min

•

July 09, 2019

DOI:

10.3791/59850-v

DOI:
10.3791/59850-v

•

09:10 min
July 09, 2019

•

3 Views
, , , ,
1School of Life Sciences,South China Normal University, 2The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

Summary

Automatically generated

Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.

Explore More Videos

AxolotlSpinal CordNeural Stem CellsGene Knock-outElectroporationCAS9GRNARegenerationMolecular MechanismsNeural Stem Cell FunctionSpinal Cord InjuryAgarose Electroporation PlatePoring PulseTransfer Pulse

Read Article

Cite this Article

Lou, W. P., Wang, L., Long, C., Liu, L., Fei, J. Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes. J. Vis. Exp. (149), e59850, doi:10.3791/59850 (2019).

Download .ris file

Copy

Share Video

.

Copy