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Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Neuroscience
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JoVE Journal Neuroscience
Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

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12,918 Views

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05:45 min

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April 26, 2019

DOI:

10.3791/58799-v

DOI:
10.3791/58799-v

•

05:45 min
April 26, 2019

•

13 Views
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1Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Summary

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This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.

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Hirschsprung’s DiseaseImmunostainingRectal Suction BiopsyCalretininS100 ProteinProtein Gene Product 9.5DiagnosisSensitivitySpecificityParaformaldehydeDehydrationParaffin EmbeddingMicrotomeAntigen RetrievalHydrogen PeroxideBovine Serum AlbuminPrimary AntibodyPolymer EnhancerSecondary Antibody

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Chi, S., Fang, M., Li, K., Yang, L., Tang, S. Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5. J. Vis. Exp. (146), e58799, doi:10.3791/58799 (2019).

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