This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.
Vizcardo, R., Rafiqul Islam, S., Maeda, T., Tamaoki, N., Good, M. L., Klemen, N. D., Bosch-Marce, M., Jia, L., Kruhlak, M. J., Restifo, N. P. A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants. J. Vis. Exp. (150), e58672, doi:10.3791/58672 (2019).