A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Raul Vizcardo; S.M. Rafiqul Islam; Takuya Maeda; Naritaka Tamaoki; Meghan L. Good; Nicholas D. Klemen; Marta Bosch-Marce; Li Jia; Mikhael J. Kruhlak; Nicholas P. Restifo

doi:10.3791/58672

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A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

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A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

6,898 Views

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10:44 min

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August 09, 2019

DOI:

10.3791/58672-v

DOI:

10.3791/58672-v

•

10:44 min

August 09, 2019

•

2 Views

Raul Vizcardo*^1,2, S.M. Rafiqul Islam*^1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Meghan L. Good^1,2, Nicholas D. Klemen^1,2, Marta Bosch-Marce^1,2, Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo^1,2

¹Surgery Branch,National Cancer Institute, NIH, ²Center for Cell-Based Therapy,National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research,National Cancer Institute, NIH

Özet

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

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A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

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