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マウス誘導多能性幹細胞由来腫瘍抗原特異的胸腺移民を生成する三次元胸腺培養システム
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
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マウス誘導多能性幹細胞由来腫瘍抗原特異的胸腺移民を生成する三次元胸腺培養システム

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6,913 Views

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10:44 min

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August 09, 2019

DOI:

10.3791/58672-v

DOI:
10.3791/58672-v

•

10:44 min
August 09, 2019

•

2 Views
, , , , , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research,National Cancer Institute, NIH

Summary

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本論文では、三次元(3D)胸腺培養系により腫瘍抗原特異的誘導多能性幹細胞由来の甲状腺系(iTE)を生成する新しい方法について説明する。iTEは、増殖、記憶形成、腫瘍抑制の能力を有するナイーブT細胞と密接に関連するT細胞の均質なサブセットである。

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Cite this Article

Vizcardo, R., Rafiqul Islam, S., Maeda, T., Tamaoki, N., Good, M. L., Klemen, N. D., Bosch-Marce, M., Jia, L., Kruhlak, M. J., Restifo, N. P. A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants. J. Vis. Exp. (150), e58672, doi:10.3791/58672 (2019).

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