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Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
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JoVE Journal Neuroscience
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

English

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7,052 Views

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06:49 min

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March 21, 2018

DOI:

10.3791/57353-v

DOI:
10.3791/57353-v

•

06:49 min
March 21, 2018

•

13 Views
, , , , , ,
1Department of Biology, MS-0314,University of Nevada, 2Integrated Neuroscience Graduate Program,University of Nevada

Summary

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This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.

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Cite this Article

Clark, D. A., Kohler, D., Mathis, A., Slankster, E., Kafle, S., Odell, S. R., Mathew, D. Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons. J. Vis. Exp. (133), e57353, doi:10.3791/57353 (2018).

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