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CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
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09:39 min
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June 09, 2018
DOI:
1Division of Molecular Neurogenetics,German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children’s Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology,Heidelberg University Hospital
Summary
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Conventional loss-of-function studies of genes using knockout animals have often been costly and time-consuming. Electroporation-based CRISPR-mediated somatic mutagenesis is a powerful tool to understand gene functions in vivo. Here, we report a method to analyze knockout phenotypes in proliferating cells of the cerebellum.
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Feng, W., Herbst, L., Lichter, P., Pfister, S. M., Liu, H., Kawauchi, D. CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. J. Vis. Exp. (136), e57311, doi:10.3791/57311 (2018).
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