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CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
1Division of Molecular Neurogenetics,German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children’s Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology,Heidelberg University Hospital
Chapters
- 00:04Title
- 01:30In Utero Electroporation
- 05:16Preparation of Cryosections from Electroporated Cerebella
- 06:22Immunostaining the Cerebellar Cryosections
- 07:38Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse
- 08:48Conclusion
Conventional loss-of-function studies of genes using knockout animals have often been costly and time-consuming. Electroporation-based CRISPR-mediated somatic mutagenesis is a powerful tool to understand gene functions in vivo. Here, we report a method to analyze knockout phenotypes in proliferating cells of the cerebellum.
