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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

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9,711 Views

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10:21 min

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February 21, 2018

DOI:

10.3791/57033-v

DOI:
10.3791/57033-v

•

10:21 min
February 21, 2018

•

3 Views
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1Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Summary

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Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.

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Zjablovskaja, P., Danek, P., Kardosova, M., Alberich-Jorda, M. Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (132), e57033, doi:10.3791/57033 (2018).

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