A highly pure population of megakaryocytes can be obtained from cord blood-derived CD34+ cells. A method for CD34+ cell isolation and megakaryocyte differentiation is described here.
Perdomo, J., Yan, F., Leung, H. H., Chong, B. H. Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells. J. Vis. Exp. (130), e56420, doi:10.3791/56420 (2017).