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Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

19,162 Views

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09:46 min

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December 27, 2017

DOI:

10.3791/56420-v

DOI:
10.3791/56420-v

•

09:46 min
December 27, 2017

•

17 Views
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1Haematology Research Unit, St George and Sutherland Clinical School,University of New South Wales, 2Haematology Department,St George and Sutherland Hospitals

Summary

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A highly pure population of megakaryocytes can be obtained from cord blood-derived CD34+ cells. A method for CD34+ cell isolation and megakaryocyte differentiation is described here.

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Perdomo, J., Yan, F., Leung, H. H., Chong, B. H. Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells. J. Vis. Exp. (130), e56420, doi:10.3791/56420 (2017).

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