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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
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October 02, 2017
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A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.
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Carbohydrate TransportSubstrate-binding ProteinStreptococcus PneumoniaeBiochemical CharacterizationStructural CharacterizationBuffer StabilityOligomerizationCrystallizationSize-exclusion ChromatographyMulti-angle Light ScatteringPre-crystallization TestingProtein ConcentrationCrystallization Screening
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Culurgioni, S., Tang, M., Hall, D. R., Walsh, M. A. Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092. J. Vis. Exp. (128), e56294, doi:10.3791/56294 (2017).
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