Journal
/
Developmental Biology
/
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

English

Automatically Generated

9,179 Views

•

12:28 min

•

November 10, 2017

DOI:

10.3791/56113-v

DOI:
10.3791/56113-v

•

12:28 min
November 10, 2017

•

2 Views
, , , , , , , ,
1Center for Microscopy and Image Analysis,University of Zurich, 2Institute for Molecular Life Sciences,University of Zurich, 3Institute for Medical Genetics,University of Zurich

Summary

Automatically generated

This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.

Explore More Videos

Super-resolution MicroscopyElectron MicroscopyProtein LocalizationZebrafish RetinaTokuyasu SectionsPlatinum ShadowingGelatin EmbeddingSucrose CryoprotectionCryo-ultramicrotomy

Read Article

Cite this Article

Mateos, J. M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S. C., Kaech, A., Bachmann-Gagescu, R., Ziegler, U. Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina. J. Vis. Exp. (129), e56113, doi:10.3791/56113 (2017).

Download .ris file

Copy

Share Video

.

Copy