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Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
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Genetics
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JoVE Journal Genetics
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

English

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12,504 Views

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11:11 min

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December 17, 2016

DOI:

10.3791/54787-v

DOI:
10.3791/54787-v

•

11:11 min
December 17, 2016

•

3 Views
, , , , , ,
1Department of Cardiology,Boston Children’s Hospital, 2Department of Pediatrics,Harvard Medical School, 3Department of Genetics,Harvard Medical School, 4Howard Hughes Medical Institute

Summary

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In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

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Cite this Article

Ding, J., Lin, Z., Jiang, J., Seidman, C. E., Seidman, J. G., Pu, W. T., Wang, D. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (118), e54787, doi:10.3791/54787 (2016).

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