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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

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06:48 min

September 15, 2016

DOI:

06:48 min
September 15, 2016

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Summary

Automatically generated

The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.

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