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Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
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Developmental Biology
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JoVE Journal Developmental Biology
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

English

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20,534 Views

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09:34 min

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July 30, 2016

DOI:

10.3791/54219-v

DOI:
10.3791/54219-v

•

09:34 min
July 30, 2016

•

8 Views
, , , , , ,
1Department of Plant and Wildlife Sciences,Brigham Young University, 2Department of Entomology,Cornell University, 3Department of Molecular Biology and Genetics,Cornell University, 4Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, 5Biological Sciences,SUNY Oswego

Summary

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A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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Koyle, M. L., Veloz, M., Judd, A. M., Wong, A. C., Newell, P. D., Douglas, A. E., Chaston, J. M. Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. J. Vis. Exp. (113), e54219, doi:10.3791/54219 (2016).

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