Journal
/
Immunology and Infection
/
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

English

Automatically Generated

10,527 Views

•

10:29 min

•

July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

•

10:29 min
July 06, 2016

•

1 Views
, ,
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Summary

Automatically generated

Investigating the interactions between bacterial pathogens and their hosts is an important area of biological research. Here, we describe the necessary techniques to measure effector translocation by Coxiella burnetii during siRNA gene silencing using BlaM substrate.

Explore More Videos

FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

Read Article

Cite this Article

Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

Download .ris file

Copy

Share Video

.

Copy