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Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

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10,135 Views

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11:06 min

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March 01, 2016

DOI:

10.3791/53802-v

DOI:
10.3791/53802-v

•

11:06 min
March 01, 2016

•

6 Views
, ,
1Department of Chemistry & Biochemistry,Fairfield University

Summary

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This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

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Cite this Article

Harper-Leatherman, A. S., Pacer, E. R., Kosciuszek, N. D. Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity. J. Vis. Exp. (109), e53802, doi:10.3791/53802 (2016).

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