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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Developmental Biology
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JoVE Journal Developmental Biology
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

English

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7,692 Views

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09:04 min

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January 30, 2017

DOI:

10.3791/53754-v

DOI:
10.3791/53754-v

•

09:04 min
January 30, 2017

•

3 Views
, , , , ,
1Department of Agricultural, Food and Nutritional Science,University of Alberta, 2Livestock Research Branch,Alberta Agriculture and Forestry, 3Laboratory of Functional Genomics of Early Embryonic Development,Université Laval

Summary

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Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA.

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Cite this Article

Salehi, R., Tsoi, S. C., Colazo, M. G., Ambrose, D. J., Robert, C., Dyck, M. K. Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform. J. Vis. Exp. (119), e53754, doi:10.3791/53754 (2017).

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