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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

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09:04 min

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January 30, 2017

DOI:

10.3791/53754-v

DOI:
10.3791/53754-v

•

09:04 min
January 30, 2017

•

2 Views
, , , , ,
1Department of Agricultural, Food and Nutritional Science,University of Alberta, 2Livestock Research Branch,Alberta Agriculture and Forestry, 3Laboratory of Functional Genomics of Early Embryonic Development,Université Laval

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Transcriptome ProfilingBovine Pre-implantation EmbryosTwo-color MicroarrayRNA ExtractionColumn-based PurificationPico-scale RNALysis BufferConditioning BufferEthanolWash BuffersDNase TreatmentElution Buffer

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Salehi, R., Tsoi, S. C., Colazo, M. G., Ambrose, D. J., Robert, C., Dyck, M. K. Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform. J. Vis. Exp. (119), e53754, doi:10.3791/53754 (2017).

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