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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Chapters
- 00:05Title
- 01:05Isolation of Total RNA
- 03:29RNA Amplification and Labeling
- 05:05Hybridization and Analysis
- 07:17Results: Two-color Microarray Analysis of Day 7 Bovine Embryos
- 08:11Conclusion
Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA.
