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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

English

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9,671 Views

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12:14 min

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March 09, 2016

DOI:

10.3791/53611-v

DOI:
10.3791/53611-v

•

12:14 min
March 09, 2016

•

2 Views
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1Department of Microbiology,Southern California Costal Water Research Project Authority

Summary

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This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

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Duplex Digital PCR AssayEnterococcus Spp.HF183 MarkerFecal ContaminationWater Quality MonitoringQPCRMaster MixDroplet GenerationPositive ControlsNo Template Controls (NTCs)

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Cao, Y., Raith, M. R., Griffith, J. F. A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters. J. Vis. Exp. (109), e53611, doi:10.3791/53611 (2016).

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