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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Medicine
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JoVE Journal Medicine
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

English

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18,994 Views

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11:27 min

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February 07, 2015

DOI:

10.3791/52350-v

DOI:
10.3791/52350-v

•

11:27 min
February 07, 2015

•

7 Views
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1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine,Wake Forest School of Medicine, 2Seahorse Biosciences

Summary

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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

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Cite this Article

Bharadwaj, M. S., Tyrrell, D. J., Lyles, M. F., Demons, J. L., Rogers, G. W., Molina, A. J. A. Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy. J. Vis. Exp. (96), e52350, doi:10.3791/52350 (2015).

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