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Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

English

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15,767 Views

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09:14 min

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June 13, 2014

DOI:

10.3791/51646-v

DOI:
10.3791/51646-v

•

09:14 min
June 13, 2014

•

2 Views
, , ,
1Department of Medicine,New York University School of Medicine, 2Departments of Pathology,New York University School of Medicine

Summary

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Static adhesion assay is a powerful tool that can be used to model the interactions between T lymphocytes and other cell types. Interactions are generated by injecting labeled T cells into wells coated with adhesion molecules, while a plate reader is used to quantify the number of adherent cells following serial washes.

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Cite this Article

Strazza, M., Azoulay-Alfaguter, I., Pedoeem, A., Mor, A. Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes. J. Vis. Exp. (88), e51646, doi:10.3791/51646 (2014).

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