Static adhesion assay is a powerful tool that can be used to model the interactions between T lymphocytes and other cell types. Interactions are generated by injecting labeled T cells into wells coated with adhesion molecules, while a plate reader is used to quantify the number of adherent cells following serial washes.
Strazza, M., Azoulay-Alfaguter, I., Pedoeem, A., Mor, A. Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes. J. Vis. Exp. (88), e51646, doi:10.3791/51646 (2014).