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Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
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Immunology and Infection
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JoVE Journal Immunology and Infection
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

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23,587 Views

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12:18 min

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November 06, 2013

DOI:

10.3791/51112-v

DOI:
10.3791/51112-v

•

12:18 min
November 06, 2013

•

12 Views
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai

Summary

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Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface proteins.

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Baculovirus Expression SystemRecombinant HemagglutininRecombinant NeuraminidaseH7N9 Influenza VirusGlycoproteinsImmunological AssaysVaccine StandardizationAntibody DiscoveryNA Enzymatic FunctionAntiviralsRecombinant Vaccine

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Margine, I., Palese, P., Krammer, F. Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System. J. Vis. Exp. (81), e51112, doi:10.3791/51112 (2013).

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