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In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

English

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13,026 Views

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10:51 min

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November 25, 2013

DOI:

10.3791/50905-v

DOI:
10.3791/50905-v

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10:51 min
November 25, 2013

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2 Views
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1Wolfson Centre for Age-Related Diseases,King’s College London, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Summary

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Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

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Cite this Article

Sonego, M., Zhou, Y., Oudin, M. J., Doherty, P., Lalli, G. In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices. J. Vis. Exp. (81), e50905, doi:10.3791/50905 (2013).

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