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February 17, 2014
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We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.
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Verchère, A., Dezi, M., Broutin, I., Picard, M. In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa. J. Vis. Exp. (84), e50894, doi:10.3791/50894 (2014).
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