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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Chemistry
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JoVE Journal Chemistry
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

English

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11,576 Views

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08:50 min

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October 10, 2013

DOI:

10.3791/50726-v

DOI:
10.3791/50726-v

•

08:50 min
October 10, 2013

•

1 Views
, , , ,
1Department of Chemistry,Tufts University, 2Department of Analytical Chemistry,Complutense University (Spain), 3Department of Electrical and Computer Engineering,Tufts University

Summary

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We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.

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MultiplexedFluorescentMicroarraySalivary ProteinAnalysisPolymer MicrospheresFiber-optic BundlesImmunoassaySuspension ArrayEncodingFluorescence MicroscopySandwich AssayBiotinylated Detection AntibodiesStreptavidinR-phycoerythrin

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Cite this Article

Nie, S., Benito-Peña, E., Zhang, H., Wu, Y., Walt, D. R. Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles. J. Vis. Exp. (80), e50726, doi:10.3791/50726 (2013).

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