このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
11,576 Views
•
08:50 min
•
October 10, 2013
DOI:
概要
Automatically generated
We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.
Read Article
Cite this Article
Nie, S., Benito-Peña, E., Zhang, H., Wu, Y., Walt, D. R. Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles. J. Vis. Exp. (80), e50726, doi:10.3791/50726 (2013).
Copy