Journal
/
Immunology and Infection
/
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

English

Automatically Generated

39,704 Views

•

17:35 min

•

November 01, 2013

DOI:

10.3791/50671-v

DOI:
10.3791/50671-v

•

17:35 min
November 01, 2013

•

3 Views
, , ,
1Department of Molecular and Biomedical Sciences,University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering,University of Maine, Orono

Summary

Automatically generated

Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

Explore More Videos

Microplate AssayRBL-2H3 Mast CellsMast Cell DegranulationTriclosanAllergic Mediatorsβ-hexosaminidaseHigh-throughput ScreeningOrganic Solvent-freeUV-Vis Spectrophotometry

Read Article

Cite this Article

Weatherly, L. M., Kennedy, R. H., Shim, J., Gosse, J. A. A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent. J. Vis. Exp. (81), e50671, doi:10.3791/50671 (2013).

Download .ris file

Copy

Share Video

.

Copy