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Immunology and Infection

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JoVE Journal Immunology and Infection
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation
 

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Article DOI: 10.3791/50671-v • 17:35 min • November 1st, 2013
November 1st, 2013 •
*1,2, *1,2, 1, 1,2
1Department of Molecular and Biomedical Sciences, University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono
* These authors contributed equally

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Summary
Automatic Translation

Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

Tags

Microplate Assay Chemical Effects RBL-2H3 Mast Cells Degranulation Triclosan Organic Solvent Allergic Disease Immune Defense Allergic Mediators Drugs Toxicants Human Health Mast Cell Function Rat Basophilic Leukemia Mast Cells Granule Component β-hexosaminidase Histamine Fluorogenic Substrate Fluorescence Intensity High-throughput Studies Antibacterial Agent Consumer Products Therapeutic Aid Allergic Skin Disease
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