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A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
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JoVE Journal 免疫学と感染
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

39,714 Views

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17:35 min

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November 01, 2013

DOI:

10.3791/50671-v

DOI:
10.3791/50671-v

•

17:35 min
November 01, 2013

•

3 Views
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1Department of Molecular and Biomedical Sciences,University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering,University of Maine, Orono

概要

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Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

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Microplate AssayRBL-2H3 Mast CellsMast Cell DegranulationTriclosanAllergic Mediatorsβ-hexosaminidaseHigh-throughput ScreeningOrganic Solvent-freeUV-Vis Spectrophotometry

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Cite this Article

Weatherly, L. M., Kennedy, R. H., Shim, J., Gosse, J. A. A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent. J. Vis. Exp. (81), e50671, doi:10.3791/50671 (2013).

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