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Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

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23,097 Views

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08:33 min

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July 10, 2011

DOI:

10.3791/2834-v

DOI:
10.3791/2834-v

•

08:33 min
July 10, 2011

•

3 Views
, , , ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Sue and Bill Gross Stem Cell Center,University of California, Irvine, 3Institute for Immunology,University of California, Irvine

Summary

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The transplantation of mouse neural stem cells (NSCs) into the spinal cords of mice with established demyelination is detailed. The preparation of NSCs, the laminectomy of thoracic vertebra 9 (T9), and transplantation of NSCs is outlined along with the pre- and post-operative care of the mice.

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Surgical TransplantationMouse Neural Stem CellsSpinal CordsMice InfectedNeurotropic Mouse Hepatitis VirusJHM StrainDemyelinating DiseaseMultiple SclerosisTransplantation Of NSCsRemyelinationClinical OutcomeCell Replacement TherapiesChronic Neurologic DiseasesIn Vivo ModelsEngrafted CellsAdapted MethodI) Preparation Of NSCsIi) Pre-operative Care Of MiceIii) Exposure Of The Spinal Cord Via LaminectomyIv) Stereotactic Injection Of NSCsIv) Post-operative Care

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Cite this Article

Carbajal, K. S., Weinger, J. G., Whitman, L. M., Schaumburg, C. S., Lane, T. E. Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus. J. Vis. Exp. (53), e2834, doi:10.3791/2834 (2011).

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