Journal
/
Neuroscience
/
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

English

Automatically Generated

15,289 Views

•

07:29 min

•

May 25, 2011

DOI:

10.3791/2681-v

DOI:
10.3791/2681-v

•

07:29 min
May 25, 2011

•

10 Views
, , , ,
1Department of Neurology,Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology,Brigham and Women’s Hospital, 3Department of Pathology,Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology,Brigham and Women’s Hospital

Summary

Automatically generated

A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.

Explore More Videos

Neural Stem CellsNSCsNeuroepitheliumCortical PlateIntermediate ProgenitorsNeuronal CellsGlial CellsCerebral CortexNormal Human NSC DevelopmentNeurological DisordersProgenitor ProliferationMigrationDifferentiationDown Syndrome NSCsAlzheimer’s Disease PhenotypeGenes Located On Human Chromosome 21DissectionCell SortingPlatingPassaging Of Human NSCsDifferentiation Of Human NSCs

Read Article

Cite this Article

Lu, J., Delli-Bovi, L. C., Hecht, J., Folkerth, R., Sheen, V. L. Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue. J. Vis. Exp. (51), e2681, doi:10.3791/2681 (2011).

Download .ris file

Copy

Share Video

.

Copy