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Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
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JoVE Journal Neurowissenschaften
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

15,289 Views

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07:29 min

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May 25, 2011

DOI:

10.3791/2681-v

DOI:
10.3791/2681-v

•

07:29 min
May 25, 2011

•

10 Views
, , , ,
1Department of Neurology,Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology,Brigham and Women’s Hospital, 3Department of Pathology,Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology,Brigham and Women’s Hospital

Summary

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A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.

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Cite this Article

Lu, J., Delli-Bovi, L. C., Hecht, J., Folkerth, R., Sheen, V. L. Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue. J. Vis. Exp. (51), e2681, doi:10.3791/2681 (2011).

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