A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.
Lu, J., Delli-Bovi, L. C., Hecht, J., Folkerth, R., Sheen, V. L. Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue. J. Vis. Exp. (51), e2681, doi:10.3791/2681 (2011).