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Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

English

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14,544 Views

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07:12 min

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November 24, 2010

DOI:

10.3791/2363-v

DOI:
10.3791/2363-v

•

07:12 min
November 24, 2010

•

6 Views
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1Microbiology, Immunology, and Pathology,Colorado State University

Summary

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Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.

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Phillips, A., Mossel, E., Sanchez-Vargas, I., Foy, B., Olson, K. Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors. J. Vis. Exp. (45), e2363, doi:10.3791/2363 (2010).

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