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Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Immunology and Infection
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JoVE Journal Immunology and Infection
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

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12,818 Views

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09:10 min

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October 18, 2010

DOI:

10.3791/2308-v

DOI:
10.3791/2308-v

•

09:10 min
October 18, 2010

•

6 Views
,
1Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Summary

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This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).

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Adhesive-tape-based SamplingFluorescence In Situ HybridizationRapid DetectionSalmonellaFresh ProduceCulture-independent DetectionCell-charged TapesSolid-phase EnrichmentLiquid Surface MinicultureDNA Probe CocktailHybridizationFluorescence Microscopy

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Cite this Article

Bisha, B., Brehm-Stecher, B. F. Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce. J. Vis. Exp. (44), e2308, doi:10.3791/2308 (2010).

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