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Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
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November 06, 2010
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We provide a reproducible method for culturing confluent monolayers of human fetal retinal pigment epithelial cells (hfRPE) cells that exhibit morphology, physiology, polarity, and protein and gene expression patterns of adult native tissue. This work has been extended to an animal model of several eye diseases.
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Retinal Pigment EpitheliumPhysiologyPathophysiologyCell Culture ProcedurePrimary Human Fetal Retinal Pigment EpitheliumHfRPE Cell CulturesPigmentationElectron MicroscopyApical Membrane MicrovilliTight Junction ProteinsEpithelial PolarityFunctionMammalian ModelsTherapeutic InterventionsAnimal ModelsEye DiseaseAbnormal Fluid AccumulationSubretinal SpacePhotoreceptor Outer SegmentsRetinal AttachmentsRPE/retina Interactions
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Maminishkis, A., Miller, S. S. Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology. J. Vis. Exp. (45), e2032, doi:10.3791/2032 (2010).
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