This content is Free Access.
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
Chapters
- 00:00Title
- 01:08Introduction
- 02:13Background on Retinal Pigment Epithelium (RPE)
- 03:26Eye Dissection
- 05:57Isolation and Dissociation of Human Fetal Retinal Pigment Epithelium (hfRPE)
- 08:07Conclusion
We provide a reproducible method for culturing confluent monolayers of human fetal retinal pigment epithelial cells (hfRPE) cells that exhibit morphology, physiology, polarity, and protein and gene expression patterns of adult native tissue. This work has been extended to an animal model of several eye diseases.
Tags
Retinal Pigment EpitheliumPhysiologyPathophysiologyCell Culture ProcedurePrimary Human Fetal Retinal Pigment EpitheliumHfRPE Cell CulturesPigmentationElectron MicroscopyApical Membrane MicrovilliTight Junction ProteinsEpithelial PolarityFunctionMammalian ModelsTherapeutic InterventionsAnimal ModelsEye DiseaseAbnormal Fluid AccumulationSubretinal SpacePhotoreceptor Outer SegmentsRetinal AttachmentsRPE/retina Interactions
