This content is Free Access.
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
19,010 Views
•
08:28 min
•
November 06, 2010
DOI:
概要
Automatically generated
We provide a reproducible method for culturing confluent monolayers of human fetal retinal pigment epithelial cells (hfRPE) cells that exhibit morphology, physiology, polarity, and protein and gene expression patterns of adult native tissue. This work has been extended to an animal model of several eye diseases.
Explore More Videos
Retinal Pigment EpitheliumPhysiologyPathophysiologyCell Culture ProcedurePrimary Human Fetal Retinal Pigment EpitheliumHfRPE Cell CulturesPigmentationElectron MicroscopyApical Membrane MicrovilliTight Junction ProteinsEpithelial PolarityFunctionMammalian ModelsTherapeutic InterventionsAnimal ModelsEye DiseaseAbnormal Fluid AccumulationSubretinal SpacePhotoreceptor Outer SegmentsRetinal AttachmentsRPE/retina Interactions
Read Article
Cite this Article
Maminishkis, A., Miller, S. S. Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology. J. Vis. Exp. (45), e2032, doi:10.3791/2032 (2010).
Copy