JOVE JOURNAL

Biochemistry

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Journal (Biochemistry)

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Computational Alanine Scanning Mutagenesis of Protein-Peptide Interface

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Sequence Diversification of Protein-Peptide Interface with Pepspec

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

Measuring Biomolecular Interactions with Weaker Affinities Using Mass Photometry and Microfluidics

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low-Affinity Protein-Protein Interactions

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez¹

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

Yeast Cell Preparation for FRET Assay for Hyperosmotic Stress Analysis

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez¹

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

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